稻瘟病抗性基因对安徽省稻瘟病菌种群抗性的影响

doi:10.3969/j.issn.1006-8082.2020.01.011

摘要/Abstract

摘要：

为分析抗稻瘟病基因对安徽省稻瘟病菌株的抗性水平的影响，采用10个水稻抗稻瘟病单基因品系对安徽不同地区的稻瘟病菌株进行致病力测定，同时对201份水稻品种的抗稻瘟病基因Pi9进行扩增，结合田间抗性表现，分析抗性基因Pi9的利用价值。抗性基因 Pi9、Pik、Pizt等对安徽地区的稻瘟病菌株具有较好的抗性，抗性频率分别为66%、60%和54%，其中携带Pi9抗性基因的水稻品种的田间抗性表现也较好，说明Pi9可直接作为分子标记辅助安徽地区水稻品种选育抗原基因。

关键词: 水稻, 稻瘟病菌, 致病力, 抗性基因

Abstract:

The virulence of rice blast resistant strains in different areas of Anhui province was determined by using 10 single gene strains of rice blast resistant. At the same time, the resistance gene Pi9 was amplified in 201 rice varieties, and its application value was analyzed in combination with the field resistance. The results showed that the resistance genes Pi9, Pik and Pizt had good resistance to the rice blast strains in Anhui province, and the resistance frequency was 66%, 60% and 54%, respectively. Field resistance of rice varieties carrying Pi9 resistance gene was also better, which indicating that Pi9 could be directly used as an antigen gene in molecular marker assisted selection breeding in Anhui province.

Key words: Oryza sativa, Magnaporthe oryzae, virulence pathogenicity, resistance genes

中图分类号:

张海珊,杨雪,章东方,严丹侃,顾江涛,张爱芳*. 稻瘟病抗性基因对安徽省稻瘟病菌种群抗性的影响[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2020.01.011 .

参考文献

[1]    OU S H. Rice Diseases[M]. 2nd. Kew Surrey：Commonwealth Mycological Institute, 1985: 109-201.
[2]    SKAMNIOTI P, GURR S J. Against the grain：safeguarding rice from rice blast disease[J]. Trends Biotechnol, 2009, 27: 141-150.
[3]    ZEIGLER R S, TOHME J, NELSON R, et al． Lineage exclusion: a proposal for linking blast population analysis to resistance breeding[C]//ZEIGLER R S, LEONG S A, TENG P S. Rice Blast Diseases. Wallingford: CAB International, 1994: 267-292.
[4]    郝鲲，马建，程治军，等．水稻抗稻瘟病基因资源与分子育种策略[J]. 植物遗传资源学报，2013，14（3）：479-485.
[5]    沈瑛，朱培良，袁筱萍，等. 中国稻瘟病菌的遗传多样性[J]. 植物病理学报，1993，23（4）：309-313.
[6]    吴建利，庄杰云，李德葆，等. 水稻对稻瘟病抗性的分子生物学研究进展[J]. 中国水稻科学，1999，13（2）：123-128.
[7]    DONG L Y, LIU S F, XU P, et al． Fine mapping of Pi57（t） conferring broad spectrum resistance against Magnaporthe oryzae in introgression line IL-E1454 derived from Oryza longistaminata [J]. Plos One, 2017, 12（10）: e0186201.
[8]    ASHIKAWA I, HAYASHI N, YAMANE H, et al．Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance [J]. Genetics, 2008, 180: 2 267-2 276.
[9]    BRYAN G T, WU K S, FARRALL L, et al． A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pita [J]. Plant Cell, 2000, 12: 2 033-2 046.
[10] CESARI S, THILLIEZ G, RIBOT C, et al. The rice resistance protein pair RGA4/RGA5 recognizes the Magnaporthe oryzae effectors AVR-Pia and AVR1-CO39 by direct binding [J]. Plant Cell, 2013, 25: 1 463-1 481.
[11] CHAUHAN R S, FARMAN M L, ZHANG H B. Genetic and physical mapping of a rice blast resistance locus， Pi-CO39（t）, that corresponds to the a virulence gene AVR1-CO39 of Magnaporthe grisea [J]． Mol Genet Genom, 2002, 267: 603-612.
[12] CHEN X W, SHANG J J, CHEN D X, et al. A B-lectin receptor kinase gene conferring rice blast resistance [J]. Plant J, 2006, 46: 794-804.
[13] FUKUOKA S, SAKA N, KOGA H, et al. Loss of function of a proline-containing protein confers durable disease resistance in rice[J]. Science, 2009, 325: 998-1 001.
[14] HAYASHI K, YOSHIDA H. Refunctionalization of the ancient rice blast disease resistance gene Pit by the recruitment of a retrotransposon as a promoter [J]. Plant J, 2009, 57: 413-425.
[15] HAYASHI N, INOUE H, KATO T, et al. Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication [J]. Plant J, 2010, 64: 498-510.
[16] HUA L X, WU J Z, CHEN C X, et al. The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast [J]. Theor Appl Genet, 2012, 125: 1 047-1 055.
[17] LEE S K, SONG M Y, SEO Y S, et al． Rice Pi5-mediated resistance to Magnaporthe oryzae requires the presence of two coiled-coil-nucleotide-binding-leucine-rich repeat genes[J]. Genetics, 2009, 181: 1 627-1 638.
[18] LI W T, ZHU Z W, CHERN M, et al．A natural allele of a transcription factor in rice confers broad-spectrum blast resistance [J]. Cell, 2017, 170: 114-126.
[19] LIN F, CHEN S, QUE Z Q, et al. The blast resistance gene Pi37 encodes a nucleotide binding site leucine-rich repeat protein and is a member of a resistance gene cluster on rice chromosome 1 [J]. Genetics, 2007, 177: 1 871-1 880.
[20] LIU X Q, LIN F, WANG L, et al． The in silico map-based cloning of Pi36, a rice coiled-coil nucleotide-binding site leucine-rich repeat gene that confers race-specific resistance to the blast fungus [J]. Genetics, 2007, 176: 2 541-2 549.
[21] LIU Y, LIU B, ZHU X Y, et al． Fine-mapping and molecular marker development for Pi56（t）, a NBS-LRR gene conferring broad-spectrum resistance to Magnaporthe oryzae in rice [J]. Theor Appl Genet, 2013, 126：985-998.
[22] MA J, LEI C, XU X, et al． Pi64, encoding a novel CC-NBS-LRR protein, confers resistance to leaf and neck blast in rice [J]. Mol Plant-microbe In, 2015, 28: 558-568.
[23] QU S H, LIU G F, ZHOU B, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice [J]. Genetics, 2006, 172（3）:1 901-1 914.
[24] SHANG J J, TAO Y, CHEN X W, et al. Identification of a new rice blast resistance gene, Pid3, by genomewide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes [J]. Genetics, 2009, 182: 1 303-1 311.
[25] SHARMA T R, MADHAV M S, SINGH B K, et al. High-resolution mapping, cloning and molecular characterization of the Pik（h） gene of rice, which confers resistance to Magnaporthe grisea [J]. Mol Genet Genom, 2005, 274（6）: 569-578.
[26] TAKAHASHI A, HAYASHI N, MIYAO A, et al. Unique features of the rice blast resistance Pish locus revealed by large scale retrotransposon-tagging [J]. BMC Plant Biol, 2010, 10: 175.
[27] WANG Z X, YANO M, YAMANOUCHI U, et al．The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes [J]. Plant J, 1999, 19: 55-64.
[28] XU X, HAYASHI N, WANG C T, et al. Rice blast resistance gene Pikahei-1（t）, a member of a resistance gene cluster on chromosome 4, encodes a nucleotide-binding site and leucine-rich repeat protein [J]. Mol Breeding, 2014, 34: 691-700.
[29] YUAN B, ZHAI C, WANG W J, et al． The Pik-p resistance to Magnaporthe oryzae in rice is mediated by a pair of closely linked CC-NBS-LRR genes [J]. Theor Appl Genet, 2011, 122: 1 017-1 028.
[30] ZHAI C, LIN F, DONG Z Q, et al. The isolation and characterization of Pik, a rice blast resistance gene which emerged after rice domestication [J]. New Phytol, 2011, 189: 321-334.
[31] ZHOU B, QU S H, LIU G F, et al. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea [J]. Mol Plant-microbe In, 2006, 19: 1 216-1 228.
[32] 卓晓轩，樊琳琳，安星宇，等. 云南地方品种子预44中一个新的抗稻瘟病基因的定位[J]. 中国水稻科学，2019，33（1）：12-19.
[33] 吴建利，庄杰云，柴荣耀，等. 水稻抗穗瘟基因的分子定位[J]. 植物病理学报，2000，30（2）：111-115.
[34] 李培富，史晓亮，王建飞，等. 太湖流域粳稻地方品种黑壳子粳抗稻瘟病基因的分子定位[J]. 中国水稻科学，2007，21（6）：579-584．
[35] 张锦文，谭亚玲，洪汝科，等. 高原粳稻子预44抗稻瘟病基因遗传分析和定位[J]. 中国水稻科学，2009，23（1）：31-35.
[36] ZHUANG J Y, MA W B, WU J L, et al. Mapping of leaf and neck blast resistance genes with resistance gene analog RAPD and RFLP in rice [J]． Euphytica, 2002, 128（3）: 363-370.
[37] 鄂志国，张丽靖，焦桂爱，等. 稻瘟病抗性基因的鉴定及利用进展[J]. 中国水稻科学，2008，22（5）：533-540．
[38] 陈能刚，陈惠查，阮仁超，等. 水稻抗稻瘟病种质资源的研究进展[J]. 贵州农业科学，2010，38（12）：7-10.
[39] 向聪，雷东阳，任西明，等. 水稻抗稻瘟病遗传育种研究进展[J]. 作物研究，2017，31（5）：547-552.
[40] 康美花，曹丰生，陈红萍，等. 水稻稻瘟病抗性基因研究进展及其在育种上的应用[J]. 江西农业科学，2010，22（2）：95-98.
[41] 江南，刘雄伦，戴良英，等. 水稻抗稻瘟病基因的定位与克隆研究进展[J]. 中国农学通报，2010，26（10）：270-275.
[42] 易怒安，李魏，戴良英. 水稻抗稻瘟病基因的克隆及其分子育种研究进展[J]. 分子植物育种，2015，13（7）：1 653-1 659.
[43] 刘辉，孟德龙，查日扬，等. 江苏水稻品种稻瘟病主效抗性基因鉴定及应用评价[J]. 福建农业学报，2015，30（5）：452-458.
[44] 田大刚，苏军，陈建民，等. 1092份水稻材料稻瘟病抗性鉴定及抗性标记分析[J]. 分子植物育种，2012，10（2）：214-221.
[45] 谢倩凤，郭建夫，杨仕华，等. 82份水稻种质资源的稻瘟病抗性评价与抗性基因鉴定[J]. 广东农业科学，2015（14）：9.
[46] 李刚，袁彩勇，曹奎荣，等. 544份水稻种质稻瘟病抗性鉴定及抗性基因的分布研究[J]. 中国农业大学学报，2018，23（5）：22-28.